Welcome to International Network for Natural Sciences

Paper Details

Research Paper | March 5, 2022

| Download

Qualitative and quantitative evaluation of tannins in bark extracts of some indigenous plants of Pakistan

Tahira Aziz Mughal, Uzma Abid, Zeb Saddiqe, Sofia Nosheen, Shazia Pervaiz

Key Words:

Int. J. Biosci.12(6), 78-84, June 2018

DOI: http://dx.doi.org/10.12692/ijb/20.2.1-20

Certification: IJB 2018 [Generate Certificate]


Plant barks contain more tannin than any other plant part. In the present study two extraction techniques were applied to extract tannins from bark of some indeginous plants of Pakistan. The tannins were extracted from the barks of plants by maceration & ultrasonic extracion. Folin-Ciocalteu method was conducted to measure the total phenolic content in bark extracts of some plants. Polyvinylpyrrolidone (PVPP) solution was used to bind the tannins, so that the non-tannins (NT) content value can be detected. Thus, the total tannins (TT) content was measured. Qualitative tests for the determination of the presence of polyphenols/tannins were also conducted. It was seen that highest tannin content was present in plant Cassia fistula whereas the lowest tannin content was present in Quercus dilatate.


Copyright © 2021
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Qualitative and quantitative evaluation of tannins in bark extracts of some indigenous plants of Pakistan

Association of Official Analytical Chemists. Official Methods of Analysis of the Association of Official Agricultural Chemists, 1975, 12 ed. Ed. AOAC, 1,. Washington.

Bandar H, Hijazi A, Rammal H, Hachem A, Saad Z, Badran B. 2013. Techniques for the extraction of bioactive compounds from Lebanese Utricadioica. American Journal of Phytomedicine and Clinical Therapeutics1, 507-513.

British Pharmacopoeia. 1999. The Stationary Office: London, volume I.

European Pharmacopoeia. 2002. EDQM: France.

FAO/IAEA. 2000. Division of Nuclear Techniques in Food and Agriculture, Quantification of Tannins in Tree Foliage, Vienna.

Paulo S. Farmacopéia Brasileira. 1988, Atheneu.

Horn D, Ditter W. 1982. Chromatographic study of interactions between polyvinylpyrrolidone and drugs. Journal of Pharmaceutical Sciences 71,1021-1026.

Makkar HPS, Bluemmel M, Browy NK, Becker K. 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods, Journal of the Science of Food and Agriculture61, 161–165.

Makkar HPS, Bluemmel M, Becker K. 1995. Formation of complexes between polyvinylpyrrolidone and polethylene glycol with tannins and their implications in gasproduction and true digestibility in in vitro techniques. British Journal of Nutrition73,897–913.

Musa AE, Gasmelseed GA. 2012. Characterization of Lawsonia inermis (Henna) as vegetable tanning material. Journal of Forest Products & Industries 1, 35-40.

Sadasivam S, Manickam A. 2008. Biochemical methods, 3 ed. New Age International Pvt. Ltd, New Delhi.

Sarker SD, Latif Z, Gray AI. 2005. Natural products isolation, Springer.

Soares LAL, Maia A, Oliveira AL, Petrovick, PR, Ortega GG.2006. Avaliação de Complexos Formadospor Catequinae Macromoléculas. Acta Farmacéutica Bonaerense 25, 10-6.

Tonon R, Grosso C, Hubinger M. 2011. Influence of emulsion composition and inlet air temperature on the microencapsulation of flaxseed oil by spray drying. Food Research International 44,282-289.

Ukoha PO, Cemaluk EAC, Nnamdi OL, Madus EP. 2011. Tannins and other phytochemical of the Samanaea saman pods and their antimicrobial activities. African Journal of Pure and Applied Chemistry. 5, 237-244.

Verza SG, Kreinecker MT, Reis V, Henriques AT, Ortega GG. 2007. Evaluation of analytical variables of the Folin-Ciocalteu method for the quantitation of the total tannins content using a Psidium guajava L. leaves aqueous extract as a model. Química Nova 30, 815-820.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background