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Isolation and characterization of psychrotrophic cellulolytic bacteria from landfill site under temperate climatic conditions

Basharat Hamid, Arshid Jehangir, Zahoor Ahamd Baba, Imran Khan

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Int. J. Biosci.13(5), 92-102, November 2018

DOI: http://dx.doi.org/10.12692/ijb/20.2.1-20

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In temperate regions due to lower temperatures the decomposition of cellulosic material is slow due to reduced metabolic activities of microbes. However, psychrotrophic bacteria could be isolated and used for enhanced decomposition of accumulating biodegradable municipal waste.  Hence the present study was aimed at the isolation and characterization of psychrotrophic cellulolytic bacteria with efficient enzyme activities. By following serial dilution and spread plate technique a total of 8 psychrotrophic cellulolytic bacteria were isolated on carboxy methyl cellulose agar media (CMC) at pH of 7.0 and temperature of 15°C after 48 hours. The isolates were screened for carboxymethyl cellulase (CMCase) activities qualitatively through congo red dilution assay and quantitatively through Dinitrosalicylic acid (DNS) method at different temperatures and incubation periods. Qualitative analysis revealed significant enzyme productions by isolates through formation of hydrolysis zones on CMC agar media with the isolate CB2 producing maximum hydrolysis zone diameter of 16mm after 72 hours. Quantitative CMCase analysis was in accordance with qualitative test and the isolate CB2 again showed highest CMCase activity of 2.33UmL-1.Based on morphological, biochemical and molecular characteristics (16SrRNA analysis) the isolate CB2 showed 96% similarity with Bacillus flexus. It was concluded that the study taken was a new work from the region and the isolates showed good enzyme activities at lower temperatures that could be either used for enhanced waste decomposition or could have industrial applications.


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Isolation and characterization of psychrotrophic cellulolytic bacteria from landfill site under temperate climatic conditions

Ahmad B, Nigar S, Shah SSA, Bashir S, Ali J, Yousaf S, Bangash JA. 2013.  Isolation and Identification of Cellulose Degrading Bacteria from Municipal Waste and Their Screening for Potential Antimicrobial Activity. World Applied  Science Journal 27(11), 1420-1426.

Ariffin H, Abdullah N, UmiKalsom MS, Shirai Y, Hassan MA. 2006. Production and characterisation of cellulase by Bacillus pumilus EB3: Int J Engi and Technol 3, 47-53.

Arimurti S, Nurani Y, Ardyati T, Suharjono S. 2017.Screening and Identification of Indigenous cellulolytic bacteria from Indonesian Coffee Pulp and Investigation of its Caffeine tolerance ability. Malaysian  Journal of  Microbiology 13(2), 109-116.

Baghel VS, Tripathia RD, Ramtekeb PW, Gopal KC, Dwivedia S, Jain RK, Rai UN, Singh SN. 2005. Psychrotrophic proteolytic bacteria from cold environment of Gangotri glacier, Western Himalaya, India. Enzyme Microbiology, Technology 36, 654–659.

Central Pollution Control Board (CPCB). (2010-11). “Annual review report” Central Pollution Control Board, Ministry of Environment and Forests,

Das A, Bhattacharya S, Murali L. 2010. Production of cellulase from a thermophilic Bacillus sp. isolated from cow dung. American Eurasian Journal of Agricultural & Environmental Sciences 8(6), 685-691.

Doi RH. 2008. Cellulase of mesophilic microbes: cellulosome and non–cellulosome producers. Annals of the New York Academy of  Sciences 1125, 267–279.

Egbere OJ, Okechalu JN, Zi SK, Danladi MM, Entonu EE, Yakubu E. 2017. Isolation and Characterization of Cellulolytic and Proteolytic Microorganisms Associated with Municipal Solid Wastes in Jos Metropolis, Nigeria. Journal of Microbiology Research 4(2), 12-18.

Gautam SP, Bundela PS, Pandey AK, Awasthi MK, Sarsaiya S. 2010. Composting of Municipal Solid Waste of Jabalpur City: Global Journal of Environmental Research 4(1), 43- 46.

Gijzen HJ, Lubberding HJ, Verhagen FJ, Zwart KB, Vogels GD. 1987. Application of rumen microorganisms for enhanced anaerobic degradation. Biological Wastes 22, 81-95.

Godana B, Mitra R, Singh S. 2007. Production of enzymes for Application on Animal Feeds.

Georlette D, Blaise V, Collins T, Amico SD, Gratia E, Hoyoux A, Marx JC, Sonan G, Feller G, Gerday C. 2004. Some like it cold: biocatalysis at low temperatures, FEMS Microbiology Rev. 28, 25-42.

Ghimire S, Bhattarai S, Phuyal S, Thapa B, Shrestha BG. 2016.  Isolation and Screening of Potential Cellulolytic and Xylanolytic Bacteria from Soil Sample for Degradation of Lignocellulosic Biomass. The Journal of Triglyceride Rich Llipoproteinsl Life Science 6(3), 165-169.

He j, Yang Z, Hu B, Ji X, Wei Y, Lin L, Zhang Q. 2015. Correlation of polyunsaturated fatty acids with the cold adaptation of Rhodotorula glutinis. Yeast  32, 683-690.

Khatiwada P, Ahmed J, Sohag MH, Islam K, Azad AK. 2016. Isolation, Screening and Characterization of Cellulase Producing Bacterial Isolates from Municipal Solid Wastes and Rice Straw Wastes 6(4), 1-5.

Lin Y, Tong Z. 2013. Metagenomic analysis on seasonal microbial variation of aviated sludge from a full-scale waste water treatment plant over four years. Envirn Microbiol Rep. 6(2), 49-53.

Logan NA, De Vos P, Genus I. 2009. Bacillus Cohn 1872. In: (Eds.) P.D. Vos, D. Jones, N.R. Kreig, W.Ludwig, F.A. Rainey, K.-H. Schleifer, W.B. Whitman. Bergey’s Manual of systematic Bacteriology volume 3: The Firmicutes, Springer, 21-127.

Masngut N, Manap S, Che Man R, Shaarani S. 2017. Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation. Indian J. Sci & Technol 10(7).

Morita Y, Kondoh K, Hasan Q, Sakaguchi T, Murakami Y, Yokoyama K, Tamiy E. 1997. Purification and characterization of a cold-active protease from psychrotrophic Serratia marcescens AP3801. J Am Chem Soc. 74, 1377–1383.

Miller GL. 1959.  Use of Dinitrosalysilic acid reagent for the determination of reducing sugar. Analytical chemistry 31, 426-428.

Nowak J, Florek M, Kwiatek W, Lekki J, Chevallier P, Zieba E. 2005. Composite structure of wood cells in petrified wood. Mater Science Engineering 25, 119–30.

Pokhrel B, Bashyal B, Mgar RT. 2014. Production, Purification and characterization of cellulase from Bacillus subtilis isolated from soil. Euro J Biotechnol and Biosci 2(5), 31-37.

Pourcher, AM, Sutra L, Hebe I, Moguedet G, Bollet C. Simoneau P, Gardan L. 2001.  Enumeration and Characterization of Cellulolytic Bacteria from Refuse of Landfill. FEMS Microbiology Ecology 34, 229-241.

Rahna K, Rathnan DR, Ambili M. 2011. Cellulase Enzyme Production by Streptomyces Sp Using Fruit Waste as Substrate: Austr J. Basi Appl Science 5, 1114-1118.

Rani DS, Nand K. 2000. Production of thermo stable cellulase-free xylonite by Clostridium absonum CFR-702. Process Biochemistry 36(4), 355–362.

Rasul F, Afroz A, Rashid U, Mehmood S, Sughra K, Zeeshan N. 2015. Screening and characterization of cellulase producing bacteria from soil and waste (molasses) of sugar industry. Int J Bioscience 6(3), 230-238.

Ray AK, Bairagi KS, Ghosh A, Sen SK. 2007. Optimization of fermentation conditions for cellulose production by Bacillus subtilis CY5 and Bacillus circulans TP3 isolated from fish gut: Acat Icht Et. Pist. 37, 47– 53.

Sethi S, Datta A, Gupta BL, Gupta S. 2013. Optimization of Cellulase Production from Bacteria Isolated from Soil: ISRN Biotechnology Article ID 985685, 7.

Shaikh NM, Patel AA, Mehta SA, Patel N. 2013. Isolation and Screening of Cellulolytic Bacteria Inhabiting Different Environment and Optimization of cellulase Production. Universal Journal of   Environmental Research & Technology 3(1), 39-49.

Sivakumar N, Zadjal AA, Bahry SA, Elshafie A, Eltayeb EA. 2016. Isolation and characterization of cellulolytic Bacillus licheniformis from compost. African Journal of Biotechnology 15(43), 2434-2446.

Teather RM, Wood PJ. 1982. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Applied Environmental  Microbiology 43(4), 777–780.

Venkata NRE, Divakar G, Rajesh T, Akram Ghazi, Asra Pourgharashi. 2013. Screening and  Isolation of Cellulase roducing Bacteria from dump yards of vegetable wastes. World Journal of  Pharmaceutical Research 3(1), 428-435.

Vimal J, Venu A, Joseph J. 2016. Isolation and identification of Cellulose Degrading Bacteria and Optimization of Cellulose Production. International Journal of Research  in Biosciences 5(3), 58-67.

Wood TM, Garcia-Campayo V. 1990. Enzymology of cellulose degradation,” Biodegradation 1(2), 147–161.


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