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GC-MS analysis for the potential bioactive compounds and in vitro efficacy of the rhizome extract of Curcuma longa L. from district Udalguri, Assam, India against white muscardine fungus Beauveria bassiana

Sanghamitra Saharia, Shibani Kalita, Dimpi Moni Kalita, Sanjana Sharmin, Sunayan Bardoloi

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Int. J. Biosci.20(6), 229-239, June 2022

DOI: http://dx.doi.org/10.12692/ijb/20.2.1-20

Certification: IJB 2022 [Generate Certificate]

Abstract

The purpose of the study was to evaluate the antifungal efficacy of different solvent-mediated rhizome extracts of Curcuma longa (turmeric) against the entomopathogenic fungus Beauveria bassiana, the causative agent of white muscardine disease in silkworm in vitro. Three different concentrations- 1%, 5% and 10% were prepared and tested against the fungus by agar well diffusion method. The methanolic extract was subjected to GC-MS analysis for detection of possible bioactive constituents as the extract exhibited maximum inhibition against Beauveria bassiana. The study revealed that the plant extract is effective in inhibiting the fungus and methanolic extract has shown a significantly greater zone of inhibition compared to the ethanolic and aqueous extracts. 10% methanolic extract showed maximum inhibition zone (72.95 %) followed by 10% ethanolic extract (61.53%) and 5% methanolic extract (58.23%) as compared to the standard drug clotrimazole (1% w/w). The GC-MS study detected the presence of important phytoconstituents such as ar-turmerone, α-turmerone, curlone, ß-Sesquiphellandrene, p-ethylguaiacol, dehydrozingerone, 4-vinylguaiacol, and zingiberene.Based on our findings, it can be concluded that the rhizome extract of turmeric has fungicidal properties against the fungus B. bassiana that can be the result of the synergistic action of potential bioactive constituents as detected in the GC-MS study.

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GC-MS analysis for the potential bioactive compounds and in vitro efficacy of the rhizome extract of Curcuma longa L. from district Udalguri, Assam, India against white muscardine fungus Beauveria bassiana

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