Welcome to International Network for Natural Sciences

Paper Details

Research Paper | March 5, 2022

| Download

GC-MS analysis for the potential bioactive compounds and in vitro efficacy of the rhizome extract of Curcuma longa L. from district Udalguri, Assam, India against white muscardine fungus Beauveria bassiana

Sanghamitra Saharia, Shibani Kalita, Dimpi Moni Kalita, Sanjana Sharmin, Sunayan Bardoloi

Key Words:

Int. J. Biosci.20(6), 229-239, June 2022

DOI: http://dx.doi.org/10.12692/ijb/20.2.1-20

Certification: IJB 2022 [Generate Certificate]


The purpose of the study was to evaluate the antifungal efficacy of different solvent-mediated rhizome extracts of Curcuma longa (turmeric) against the entomopathogenic fungus Beauveria bassiana, the causative agent of white muscardine disease in silkworm in vitro. Three different concentrations- 1%, 5% and 10% were prepared and tested against the fungus by agar well diffusion method. The methanolic extract was subjected to GC-MS analysis for detection of possible bioactive constituents as the extract exhibited maximum inhibition against Beauveria bassiana. The study revealed that the plant extract is effective in inhibiting the fungus and methanolic extract has shown a significantly greater zone of inhibition compared to the ethanolic and aqueous extracts. 10% methanolic extract showed maximum inhibition zone (72.95 %) followed by 10% ethanolic extract (61.53%) and 5% methanolic extract (58.23%) as compared to the standard drug clotrimazole (1% w/w). The GC-MS study detected the presence of important phytoconstituents such as ar-turmerone, α-turmerone, curlone, ß-Sesquiphellandrene, p-ethylguaiacol, dehydrozingerone, 4-vinylguaiacol, and zingiberene.Based on our findings, it can be concluded that the rhizome extract of turmeric has fungicidal properties against the fungus B. bassiana that can be the result of the synergistic action of potential bioactive constituents as detected in the GC-MS study.


Copyright © 2021
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

GC-MS analysis for the potential bioactive compounds and in vitro efficacy of the rhizome extract of Curcuma longa L. from district Udalguri, Assam, India against white muscardine fungus Beauveria bassiana

Abdel-Lateef E, Mahmoud F, Hammam O, El-Ahwany E, El-Wakil E, Kandil S, Abu-Taleb H, El-Sayed M, Hassenein H. 2016. Bioactive chemical constituents of Curcuma longa L. rhizomes extract inhibit the growth of human hepatoma cell line (HepG2). Acta Pharmaceutica 66(3), 387-398. https://10.1515/acph-2016-0028

Araujo MCP, Antunes LMG, and Takahashi CS. 2001. Protective effect of thiourea, a hydroxy/radical scavenger, on curcumin induced chromosomal abberrations in an in vitro mammalian cell system. Teratogenesis Carcinogenesis and Mutagenesis 21(2), 175-180. https://doi.org/10.1002/1520-6866(2001)21:2<175::AID-TCM6>3.0.CO;2-V

Ashraf K, Sultan S. 2017. A comprehensive review on Curcuma longa Linn. Phytochemical, pharmacological and molecular study. International Journal of Green Pharmacy 11(4), 671-685. https://doi.org/1022377/ijgp.v11i04.1343

Avanco GB, Ferreira FD, Bomfim NS, Santos PADSRD, Peralta RM, Brugnari T, Mallmann CA, Abreu Filho BAD, Mikcha JMG, Jr. Machinski M. 2017.  Curcuma longa L. essential oil composition, antioxidant effect, and effect on Fusarium verticillioides and fumonisin production. Food Control 73, 806-813. https://doi.org/10.1016/j.fppdcont.2016.09.032

Barra A, Coroneo V, Dessi S, Cabras P, Angioni A. 2010. Chemical Variability, Antifungal and Antioxidant Activity of Eucalyptus camaldulensis Essential Oil from Sardinia. Natural Product Communications 5(2), 329-335. https://doi.org/10.1177/1934578X1000500232

Chakravorty R, Das R, Neog K, Das K, Sahu MA. 2007. Diagnostic manual for diseases and pest of muga silkworm and their host plants, Published by CMER&TI, Central Silk Board, Lahdoigarh, Jorhat, Assam, 1-47.

Chatterjee S, Variyar PS, Gholap AS, Padwal-Desai SR, Bongirwar DR. 2000. Effect of γ-irradiation on the volatile oil constituents of turmeric (Curcuma longa). Food Research International 33, 103-106. https://doi.org/10.1016/S0963-9969(00)00012-0

Chowdhury H, Banerjee T, Walia S. 2008. In vitro screening of Curcuma longa L and its derivatives as antifungal agents against Helminthosporrum oryzae and Fusarium Solani. Pesticide Research Journal 20(1), 6-9.

Devi PPSV. 2015. Studies on the management of white muscardine disease in the mulberry silkworm, Bombyx mori L. Ph.D thesis, Manonmaniam Sundaranar University, Tirunelveli, India, 36-37.

Dosoky NS, Satyal P, Setzer WN. Variations in the volatile compositions of curcuma species. 2019. Foods 8(53), 1-14. https://doi.org/10.3390/foods8020053

Giordano A, Tommonaro G. 2019. Curcumin and Cancer. Nutrients 11, 1-20. https://doi.org/10.3390/nu11102376

Girija S, Duraipandiyan V, Kuppusamy PS, Gajendran H, Rajagopal R. 2014. Chromatographic Characterization and GC-MS evaluation of the Bioactive Constituents with Antimicrobial Potential from the Pigmented Ink of Loligo duvauceli. International Scholarly Research Notices 2014, 1-7. https://doi.org/10.1155/2014/820745.

Gul P, Bakht J. 2015. Antimicrobial activity of turmeric extract and its potential use in food industry. Journal of Food Science and Technology 52(4), 2272-2279. https://doi.org/10.1007/s13197-013-1195-4

Gupta SC, Patchva S, Aggarwal BB. 2012. Therapeutic Roles of Curcumin: Lessons Learned from Clinical Trials. The American Association of pharmaceutical Scientists Journal 15(1), 195-217. https://doi.org/10.1208/s12248-012-9432-8

He X, Zhang L, Chen J, Sui J, Yi G, Wu J, Ma Y. 2019 Correlation between Chemical Composiotion and Antifungal Activity of Clausena lansium Essential Oil against Candida spp. Molecules 24(7), 1-11. https://doi.org/10.3390/molecules24071394.

Jankasem M, Wuthi-Udomlert M, Gritsanapam W. 2013. Antidermatophytic Properties of Ar-Turmerone, Turmeric Oil, and Curcuma longa Preparations. International Scholar Research Notes Dermatology 2013, 1-3. https://doi.org/10.1155/2013/250597.

Jayaramaiah M, Kuberappa GC, Devaiah MC, Kotikal YK. 1986. White muscardine disease of silkworm and its management. Indian Silk 25(8), 15-16.

Saju KA, Venugopal MN, Joseph MM. 1998. Antifungal and insect-repellent activities of essential oil of turmeric (Curcuma longa L.). Current Science 75(7), 660-662.

Kapoor A. 1997. Antifungal activities of fresh juice and aqueous extracts of turmeric (Curcuma longa) and ginger (Zingiber officinale). Journal of Phytological Research 10(1-2), 59-62.

Kumar M, Pal K, Pratap P, Gour JK. 2022. Antileishmanial potential of different extracts of Curcuma longa rhizome against Leishmania donovani. Journal of Scientific Research 66(1). 129-141. https://doi.org/10.37398/JSR.2022.660114

Lampe, V. 1918. Synthesis of curcumin. Berichte der Deutschen Chemischen Gesellschaft 51, 1347-1355.

Lee HS, Choi KJ, Cho KY, Ahn YJ. 2003. Fungicidal Activity of ar-Turmerone Identified in Curcuma longa Rhizome against Six Pathogenic Journal of Applied Biological Chemistry 46(1), 25-28.

Kim MK, Choi GJ, Lee HS. 2003. Fungicidal property of Curcuma longa L. rhizome- derived curcumin against phytopathogenic fungi in a greenhouse. Journal of Agricultural Food Chemistry 51, 1578-1581. https://doi.org/10.1021/jf0210369

Murugesh J, Annigeri RG, Mangala GK, Mythily PH, Chandrakala J. 2019. Evaluation of the antifungal efficacy of different concentrations of Curcuma longa on Candida albicans. Journal of Oral and Maxillofacial Pathology 23(2), 1-6. https://doi.org/10.4103/jomfp.JOMFP_200_18

Nair A, Amalraj A, Jacob J, Kunnumakkara AB, Gopi S. 2019. Non-curcuminoids from Turmeric and Their Potential in Cancer Therapy and Anticancer Drug Delivery Formulations. Biomolecules 9(13), 1-36. https://doi.org/10.3390/biom9010013

Nakasone KK, Peterson SW, Jong SC. 2004. Preservation and distribution of fungal cultures. Biodiversity of Fungi: Inventory and Monitoring Methods. Amsterdam: Elsevier Academic Press, 37-47.

Negi PS, Jayaprakasha GK, Rao JM, Sakariah KK. 1999. Antibacterial Activity of Turmeric Oil: A Byproduct from Curcumin Manufacture. Journal Agricultural and Food Chemistry 47(10), 4297-4300. https://doi.org/10.1021/jf990308d

Okiki PA, Borke RV, Odesanya BO, Muhammad AA, Sobajo OA. 2017.  Chemical qualities of dried rhizomes of Curcuma longa Linn. And the antimicrobial activities of its extracts on microorganisms associated in skin infections. Der Pharma Chemica 9(21), 17-28.

Panchal P, Parvez N. 2019. Phytochemical analysis of medicinal herb (Ocimum sanctum). International Journal of Nanomaterials, Nanotechnology and Nanomedicine 5(2), 008-011. http://dx.doi.org/10.17352/2455-3492.000029.

Prance. GT, Chadwick DJ, Marsh J. 1994. Ethnobotany and the Search for New Drugs. Ciba Foundation Symposium, John Wiley and Sons, England, 185.

Prastiyanto M, Rohmah N, Effendi L, Arifin R, Wardoyo FA, Wilson W, Mukaromah H, Dewi SS, Darmawati S. 2021. Antifungal activities of the rhizome extract of five member Zingiberaceae against Candida Albicans and Trichophyton rubrum. Bioderversitas 22(3), 1509-1513. https://doi.org/10.13057/biodiv/d220355

Ramos Y, Taibo AD, Jimenez JA, Portal O. 2020. Endophytic establishment of Beauveria bassiana and Metarhizium anisopliae in maize plants and its effect against Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae. Egyptian Journal of Pest Control 30(20), 1-6. https://doi.org/10.1186/s41938-020-00223-2

Rubab M, Chelliah R, Saravanakumar K, Barathikannan K, Wei S, Kim JR, Yoo D, Wang MH, Oh DH. 2020. Bioactive Potential of 2-Methoxy-4-Vinylphenol and Benzofuran from Brassica Oleracea L. var. capitate f, rubra (Red Cabbage) on Oxidative and Microbiological Stability of Beef Meat. Foods 9(5), 1-21. https://doi.org/10.3390/foods9050568.

Samadi FM, Suhail S, Sonam M, Sharma N, Singh S, Gupta S, Dobhai A, Pradhan H. 2019. Antifungal efficacy of herbs. Journal of Oral Biology Craniofacial Research 9(1), 28-32. https://doi.org/10.1016/j.jobcr.2018.06.002.

Shanmugam S, Bhavani P. 2014. Studies on the comparison of phytochemical constituents and antimicrobial activity of Curcuma longa varieties. International Journal of Current Microbiology and Applied Sciences 3(9), 573-581.

Sharma M, Sharma R. 2011 Synergistic Antifungal Activity of Curcuma longa (Turmeric) and Zingiber Officinale (Ginger) Essential Oils Against Dermatophyte Infections. Journal of Essential Oil Bearing Plants 14(1), 38-47. https://doi.org/10.1080/0972060x.2011.10643899

Shekhawrat PS, Prasada R. 1991. Antifungal properties of some plant extracts: Inhibition of spore germination. Indian Phytopathology 67, 150-152.

Soni VK, Mehta A, Ratre YK, Tiwari AK, Amit A, Singh RP, Sonkar SC, Chaturvedi N, Shukla D, Vishvakarma NK. 2020. Curcumin, a traditional spice component, can hold the promise against COVID-19? European Journal of Pharmacology 886, 1-10. https://doi.org/10.1016/j.eiphar.2020.173551.

Ungphaiboon S, Supavita T, Singchangchai P, Sungkarak S, Rattanasuwan P, Itharat A. 2005. Study on antioxidant and antimicrobial activities of turmeric clear liquid soap for wound treatment of HIV patients. Songklanakarin Journal of Science and Technology 27(2), 569-578.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background