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Functional prediction of F3’5’H in color alteration in cotton: an in-silico comparative analysis between cotton and viola

Ammara Ahad, Sana Tahir, Muhammad Azam Ali, Adnan Iqbal, Mukhtar Ahmed, Sidra Akhtar, Rabia Nawaz, Abdul Qayyum Rao, Ahmad Ali Shahid, Zia Ur Rahman, Tayyab Husnain

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Int. J. Biosci.13(3), 185-197, September 2018

DOI: http://dx.doi.org/10.12692/ijb/20.2.1-20

Certification: IJB 2018 [Generate Certificate]


Anthocyanins are water-soluble pigments giving the purple, red and blue colors to various flowers and fruits. Anthocyanins are widespread amongst seed plants playing physiological roles such as to attract pollinators, seed dispersers and against UV–B protection. A key enzyme of this flavonoid pathway, Flavonoid 3’, 5’ hydoxylase,F3’5’H is involved in delphinidin production. Molecular level modifications in pigmentation pattern of cotton fiber, is an attractive and innovative area of research. In present study, comparison of F3’5’H in Gossypium hirsutum (Cotton) and Viola × wittrockiana, sequence alignment for homology, protein modeling along their structural validation and molecular docking analysis were computed. Docking studies were carried out using Autodock Vina and the ligands used were Naringenin and Quercetin. Analysis of results showed the best binding energy values of Viola F3’5’H i-e -8.9 & -9.9 with both ligands respectively. Based on these bioinformatics outcomes it was hypothesized that no matter the presence of this gene naturally in cotton, an over expression of Viola F3’5’H had a potency to alter pigmentation pattern in cotton fibers. This predictive screening method may prove to be significant for productive experimental studies and considered as a bypass for commercial scale research in terms of time and cost.


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Functional prediction of F3’5’H in color alteration in cotton: an in-silico comparative analysis between cotton and viola

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