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Evaluation of the antioxidant and antimicrobial potentials of marine invertebrates collected off Agusan del Norte, Philippines: Lobophytum baratum, Sarcophyton ehrenbergi, Isis sp., and Demospongia sp.

Ma. Karla P. Barela, Ephrime B. Metillo, Mylene M. Uy

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Int. J. Biosci.12(4), 127-133, April 2018

DOI: http://dx.doi.org/10.12692/ijb/20.2.1-20

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This study was conducted to investigate the potentials of marine invertebrates as sources of bioactive compounds. Four marine invertebrates were collected off Agusan del Norte, Philippines, and were taxonomically identified as Lobophytum baratum, Sarcophyton ehrenbergi, Isis sp., and Demospongia sp.  Polar and nonpolar extracts, prepared by extraction of the samples with 50:50 ethanol-water and 50:50 ethylactetate-methanol, respectively, were tested for their antioxidant and antimicrobial activities. None of the extracts showed strong antioxidant activity comparable to the standard ascorbic acid in the antioxidant screening using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging method. However, the polar extract of the soft coral Sarcophyton ehrenbergi showed high Ascorbic Acid Equivalents (AAE) as well as high Butylated Hydroxytoluene Equivalents (BHTE) values in the total antioxidant assay conducted using the Phosphomolybdenum method. Among the marine invertebrate extracts subjected to antimicrobial assay, the nonpolar extract of the soft coral Lobophytum baratum was shown to be the most active against Bacillus subtilis and Escherichia coli with zones of inhibition of 21.5 ± 2.1 mm and 12.7 ± 0.9 mm, respectively. However, the extracts failed to show any antifungal activity against the test organisms Sacaromyce scerevisiae and Aspergillus niger. The results indicated the possibility of obtaining antioxidant and antibacterial compounds from S. enrenbergi and L. baratum, respectively.


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