Welcome to International Network for Natural Sciences

Paper Details

Research Paper | March 5, 2022

| Download

Effect of grafting season, rootstocks and seedling age on success of stone grafting and pigments synthesis in Sindhri leaves

Sidra Kiran, Javed Iqbal, Allah Bakhsh, Hafiz Asif-ur-Rehman, Hameed Ullah, Iftikhar Ahmad, Fatma Bibi, Samad Raza, Atif Iqbal, Syed Inam Ullah Shah Bukhari, Subhan Danish

Key Words:

J. Bio. Env. Sci.13(3), 63-72, September 2018

DOI: http://dx.doi.org/10.12692/ijb/20.2.1-20

Certification: JBES 2018 [Generate Certificate]


Mango (Mangifera indica L.) is a highly cross-pollinated and allopolyploid in nature. It is mandatory to keep such fruit plants true-to-type through asexual techniques. A preliminary study was conducted with hypothesis that season and age of seedlings rootstock could be effective to improve the success rate of stone grafted seedlings and synthesis of chlorophyll in fresh leaves. For experimental purpose freshly extracted stones of commercial mango cultivars; Sindhri, SB Chaunsa, Dusehri, Sufaid Chaunsa, Anwar Retual, Retual Late No. 12 and Desi stones were planted in polyethylene bags containing the potting media to observe the performance of different age (10, 15, 20 and 25 days after emergence) seedling rootstocks with specific scion of Sindhri in three seasons (August, September and October).Results confirmed that October is the best season for Sindhri stone grafting on seedlings rootstock of Sindhri, SB Chaunsa and Sufaid Chaunsa having epicotyl age of 10 days. However, sprouting of 1st leaf in Sindhri was best where grafting was done in August. A significant improvement in chlorophyll a (75.9%), chlorophyll b (73.0%) and total chlorophyll (74.7%) validated the effectiveness of August season for stone grafting of Sindhri on seedlings of Retual Late 12. However, on the basis of highest success rate (68.3%) of Sindhri on SB Chaunsa, it is concluded that October is the best season for stone grafting of Sindhri.


Copyright © 2021
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Effect of grafting season, rootstocks and seedling age on success of stone grafting and pigments synthesis in Sindhri leaves

Adams WW, Demmig-Adams B, Rosenstiel TN, Ebbert V. 2001.Dependence of photosynthesis and energy dissipation activity upon growth form and light environment during the winter. Photosynthesis Research67(1-2), 51–62. http://dx.doi.org/10.1023/A:1010688528773

Akinnifesi FK, Sileshi G, Mkonda A, Ajayi OC, Mhango J, Chilanga T.2008. Germplasm supply, propagation and nursery management of miombo fruit trees. In:  F. K. Akinnifesi, R. R. B. Leakey, O.C. Ajayi, G. Sileshi, Z. Tchoundjeu, P. Matakala and F. Kwesiga (Eds). Indigenous fruit trees in the tropics: domestication, utilization and commercialization. CAB International Publishing, Wallingford, UK.341 p.

Alam MA, Islam MZ, Uddin JC, Quamruzzaman AK. 2006. Effect of age  of  seedling  and  variety  of  scion  in  stonegrafting  of  mango.  International Journal of Sustainable Crop Production. 2, 27-32.

Alexander DM. 1989. The mango in Australia, Common-wealth Scientific and Industrial Research Organization, Australia.1-28 p.

Anees M, Tahir FM, Shahzad J, Mahmood N. 2011. Effect of foliar application of micronutrients on the quality of mango (Mangifera indica L.) cv. Dusehri fruit. Mycopath9, 25-28.

Anwar R, Ahmad S, Yaseen M, Ahmad W, Nafees M. 2011. Bimonthly nutrient application programme on calcareous soil improves flowering and fruit set in mango (Mangifera indica L.). Pakistan Journal of Botany.43, 983–990. http://dx.doi.org/

Arnon DI. 1949. Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiology.24, 1-15. http://dx.doi.org/10.1104/pp.24.1.1

Bally ISE.2006. Mangifera indica (mango) Anacardiaceae (cashew family). Species Profiles for Pacific Island Agroforestry, p 1-25. www.traditionaltree.org

Chakrabarty U, Sadhu MK. 1989. Anatomy of graft union in epicotyl grafting of mango. Acta Horticulturae. 231,182–85.

Food and Agriculture Organization of United Nations. 2013. Faostat. http://apps.fao.org/faostat

Gagandeep K, Malhi CS. 2006. Effect of age of rootstock and growing medium on the success of epicotyl grafting in mango. Indian Journal of Horticulture63, 244–247.

Garcia R, Asins MJ, Forner J, Carbonell EA.1999. Genetic analysis of apomixes in Citrus and Poncirus by molecular markers. Theoretical and Applied Genetics.99, 511–518.

Gunjate RT. 1989. Standardization of stone grafting for the Konkan region. Acta Horticulturae. 231, 164-167.

Islam MN, Rahim MA, Naher MNA, Azad, MI Shahjahan M.2004.Effect of time of operation and age of rootstock on the success of inserted contact grafting in mango.Asian Journal of Plant Sciences. 3,636–641. http://dx.doi.org/10.3923/ajps.2004.636.641.

Kashyap R, Shrivastava SS, Sharma AB. 1989. Studies on vegetative propagation of mango. Acta Horticulturae 231, 263-265.

Leegood R. 1995. Effects of temperature on photosynthesis and photorespiration. In: N. Smirnoff (Ed). Environment and plant metabolism.Bios, Oxford.45–62 p.

Mng’omba SA, Akinnifesi FK, Sileshi G, Ajayi OC, Chakeredza S, Mwase FW. 2008. A decision support tool for propagating Miombo indigenous fruit trees of southern Africa. African Journal of Biotechnology.7 (25), 4677-4686.

Oquist G, Huner NPA. 2003. Photosynthesis of overwintering evergreen plants. Annual Review of Plant Biology 54, 329–355. http://dx.doi.org/10.1146/annurev.arplant.54.072402.115741.

Pina A, Errea P.2005. A review of new advances in mechanism of graft compatible – incompatible in Prunus spp. Scientia Horticulture 106, 1-11.

Ram S, Litz, RE. 1997. Crop production: propagation. In: R.E. Litz (Eds) The mango: botany, production and uses.CAB International. 363-400 p.

Ruiz C, Breto MP, Asins MJ.2000. A quick methodology to identify sexual seedlings in citrus breeding programs using SSR markers. Euphytica 112, 89–94.

Sadhu MK. 2005.Plant propagation.New Age International Ltd., New Delhi, India.

Steel RG, Torrie JH, Dickey DA.1997. Principles and Procedures of Statistics: A Biometrical Approach, 3rd edn. McGraw Hill Book International Co., Singapore.

Xiang C, Roose ML.1988. Frequency and characteristic of nucellar and zygotic seedlings in 12 citrus rootstocks. Scientia Horticulturae37, 47-49.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background