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Bio-chemical alterations: markers for the identification of source of resistance in brassica germplasm against white rust disease

Muhammad Asif, Muhammad Atiq, Muhammad Rizwan Bashir, Owais Yasin, Nasir Ahmad Rajput, Yasir Ali, Ahmed Subhani, Shakila Kausar, Muhammad Imran, Akhtar Hameed, Shafqat Ali

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Int. J. Biosci.13(1), 363-375, July 2018

DOI: http://dx.doi.org/10.12692/ijb/20.2.1-20

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Rapeseed (Brassica napus) is affected by a devastating disease white rust (Albugo candida) which induces change in biochemistry of Plant. Pot experiment was conducted to study biochemical activities and basic association of resistance in germplasm. Fifteen brassica varieties/lines were cultivated in pots and kept in glasshouse. After inoculation, inoculated and uninoculated leaves were collected and enzyme activity was recorded for comparison. Basic trend of catalases (CAT), phenolic, and peroxidases (POD), super oxide dismutase (SOD) and Hydrogen peroxide showed increase in activity in inoculated plants of resistant variety (Faisal Canola) while decrease in protein activity was observed in inoculated leaves when compared to uninoculated. In resistant cultivars i.e Faisal Canola activity of SOD (0.123 to 1.961mg-1 Protein), POD (0.053 to 1.028µmol H2O2mg-1 Protein), CAT (0.214 to 1.912µmol H2O2mg-1 Protein), phenolic (0.123 to 1.961mg/mg of leaves) and H2O2 (0.448 to 1.714µmol g-1 fw) increased while reduction in protein activity was observed from (3.14 to 1.416mg/g of leaves). While in a comparison to resistant variety, a very rapid increase in above mentioned parameters was observed in susceptible variety (Toria Selection A). It was concluded that biochemical alterations provide strong factual evidence and effective marker for identification of resistant germplasm.


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Bio-chemical alterations: markers for the identification of source of resistance in brassica germplasm against white rust disease

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